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Background: Tetracycline is a broad-spectrum antibiotic that is used to treat diseases caused by different types of bacteria. Objective:To estimate tetracycline hydrochloride in pharmaceuticals through coupling with a new reagent, sulfanilic acid. Patients and Methods: The proposed method for determination of tetracycline hydrochloride was applied to four different types of pharmaceutical drug companies and the results were compared with a standard method (HPLC method) to determine their compatibility and the results were satisfactory. Results: The method is based on azo coupling reaction between sulfanilic acid and tetracycline in alkaline medium. The colored compound produced by the reaction has a higher absorbance at a wavelength of 403nm. The Beer ,s law was applied in the concentration range (8.0 - 95.0μg / ml) and the detection limit, molar absorptivity and correlation coefficient were 0.2μg / ml, 6.536x103L / mol.cm and 0.9997 respectively. The method was successfully applied to the evaluation of tetracycline hydrochloride in pharmaceuticals. Conclusion:The proposed method for determination of tetracycline hydrochloride is a simple and sensitive method, where the tetracycline hydrochloride can be estimated at low concentration and accurately. This method needs a simple device and the method can be used in quality control centers. The recommended method has been successfully applied to estimate tetracycline hydrochloride in pharmaceuticals.