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<i>Brucella</i> is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of covalently immobilized Tbilisi bacteriophages as high-performing bio-receptors. We coupled our efficient SERS nano-biosensor to a Raman system to develop an on-field phage-based bio-sensing platform capable of monitoring the target bacteria. The developed biosensor allowed us to identify <i>Brucella abortus</i> in milk by our portable SERS device. Upon bacterial capture from samples (10⁴ cells), a signal related to the pathogen recognition was observed, proving the concrete applicability of our system for on-site and in-food detection.