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The complete mitochondrial genome (mitogenome) of the ambush bug, Carcinochelis bannaensis, was determined in this study. The sequenced mitogenome is a typical circular DNA molecule of 15,335 bp, containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a putative control region. All protein-coding genes initiate with ATN codons and terminate with TAA codons except for COII,COIII and ND5 use a single T residue as the stop codon. All tRNAs have the clover-leaf structure except for the tRNASer(AGN) and the length of them range from 61 to 71 bp. The control region is 797 bp long with an A + T content of 66.3%. The phylogenetic analysis result supports the monophyly of Phymatinae.