Curcumin Alleviates IUGR Jejunum Damage by Increasing Antioxidant Capacity through Nrf2/Keap1 Pathway in Growing Pigs

oleh: Enfa Yan, Jiaqi Zhang, Hongli Han, Jiamin Wu, Zhending Gan, Chengheng Wei, Lili Zhang, Chao Wang, Tian Wang

Format: Article
Diterbitkan: MDPI AG 2019-12-01

Deskripsi

The purpose of this study was to explore the effects of curcumin on IUGR jejunum damage. A total of 24 IUGR and 12 normal-birth weight (NBW) female crossbred (Duroc &#215; Landrace &#215; Large White) piglets were randomly assigned into three groups at weaning (26 days): IUGR group, NBW group, and IUGR + CUR group, which were fed diets containing 0 mg/kg (NBW), 0 mg/kg (IUGR) and 200 mg/kg (IUGR + CUR) curcumin from 26 to 115 days of age. Results showed that dietary supplementation with 200 mg/kg curcumin significantly increased the total superoxide dismutase (T-SOD) activity and decreased the malondialdehyde (MDA) content in the jejunum of IUGR pigs (<i>p</i> &lt; 0.05). Results of real-time PCR showed that the IUGR + CUR group significantly increased the gene expression of NF-E2-related factor 2 (<i>Nrf2</i>) (<i>p</i> &lt; 0.05), and increased the glutamate-cysteine ligase catalytic subunit (<i>GCLC</i>), superoxide dismutase 1 (<i>SOD1</i>), glutamate-cysteine ligase modifier subunit (<i>GCLM</i>), and NAD(P)H quinone dehydrogenase 1 (<i>NQO1</i>) mRNA expression compared with the IUGR group (<i>p</i> &lt; 0.05). Western blot results showed that dietary supplementation with 200 mg/kg curcumin significantly increased the protein levels of Nrf2 and NQO1. Compared with the IUGR group, pigs in IUGR + CUR group showed significantly decreased the levels of tumor necrosis factor-&#945; (<i>TNF&#945;</i>), interleukin-6 (<i>IL-6</i>), and interferon gamma (<i>IFN&#947;</i>) (<i>p</i> &lt; 0.05), and increased the interleukin-2 (<i>IL-2</i>) level (<i>p</i> &lt; 0.05). Dietary supplementation with 200 mg/kg curcumin significantly reduced cysteinyl aspartate specific proteinase 3 (<i>caspase3</i>), BCL2-associated X protein (<i>bax</i>), B-cellCLL/lymphoma 2 (<i>bcl2</i>), and heat-shock protein 70 (<i>hsp70</i>) mRNA expression, and increased occludin (<i>ocln</i>) mRNA expression (<i>p</i> &lt; 0.05). In conclusion, dietary supplementation with 200 mg/kg curcumin can alleviate jejunum damage in IUGR growing pigs, through Nrf2/Keap1 pathway.