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Antigenic fractions of 100, 50, 37, and 28 kDa obtained through the SDS-PAGE method that were more frequently recognized by anti-<i>Coccidioides</i> antibodies in the sera of coccidioidomycosis patients were selected using western blotting. Subsequently, these bands were sequenced, and the obtained proteins were analysed by BLAST to choose peptides specific for <i>Coccidioides</i> spp. from among the shared aligned sequences of related fungi. A peptide specific for <i>C. immitis</i> was selected from the &#8220;GPI anchored serine-threonine rich protein OS <i>C. immitis</i>&#8222;, while from the &#8220;uncharacterized protein of <i>C. immitis</i>&#8222;, we selected a peptide for <i>C. immitis</i> and <i>C. posadasii</i>. These proteins arose from the 100 kDa antigenic fraction. From the protein &#8220;fatty acid amide hydrolase 1 of <i>C. posadasii</i>&#8222; that was identified from the 50 kDa antigenic fraction, a peptide was selected that recognized <i>C. immitis</i> and <i>C. posadasii</i>. In addition, the analysis of all the peptides (353) of each of the assembled proteins showed that only 35 had 100% identity with proteins of <i>C. immitis</i> and <i>C. posadasii</i>, one had 100% identity with only <i>C. immitis</i>, and one had 100% identity with only <i>C. posadasii.</i> These peptides can be used as diagnostic reagents, vaccines, and antifungals.