Find in Library

<i>Endoclita signifer</i> is a prominent wood-boring insect species in eucalyptus plantations in Guangxi, China, causing significant ecological and economic damage. A novel approach to controlling the challenging wood-boring pest involves disrupting the olfactory communication between insects and the volatile compounds emitted by plants. To identify the olfactory proteins contributing to host selection based on 11 GC-EAD-active volatiles from eucalyptus leaves and to discover the highly expressed olfactory proteins, we conducted a study on the antennal transcriptomes of adult <i>E. signifer</i> and screened key olfactory proteins in the antennae. We identified a total of 69 olfactory proteins. When compared to the larval transcriptomes, the antennal transcriptome of adult <i>E. signifer</i> revealed the presence of 17 new odorant-binding proteins (OBPs), including 2 pheromone-binding proteins (PBPs), 7 previously unreported chemosensory proteins (CSPs), 17 new odorant receptors (ORs), 4 new gustatory receptors (GRs), 11 novel ionotropic receptors (IRs), and 2 sensory neuron membrane proteins (SNMPs). Through the phylogenetic tree of OBPs and ORs, we identified <i>EsigPBP2</i> and <i>EsigPBP3</i> as two of the three PBPs, designated <i>EsigOR13</i> as EsigOrco, and recognized <i>EsigOR10</i> and <i>EsigOR22</i> as the newly discovered EsigPRs in <i>E. signifer</i>. In the adult antennae, the expression levels of <i>EsigGOBP14</i>, <i>EsigGOBP13</i>, <i>EsigOBP14</i>, <i>EsigOBP17</i>, <i>EsigCSP14</i>, and <i>EsigOR16</i> were notably high, indicating that these proteins could be pivotal in binding to plant volatiles.