Microbiological assessment of freshly-shot wild boars meat in Lazio Region, Viterbo territory: a preliminary study
oleh: Eda Maria Flores Rodas, Tatiana Bogdanova, Teresa Bossù, Sabrina Pecchi, Francesco Tomassetti, Paola De Santis, Rita Tolli, Roberto Condoleo, Sara Greco, Alberto Brozzi, Stefano Bilei, Giuseppe Micarelli, Enrica Martini, Massimo Palazzetti
| Format: | Article |
|---|---|
| Diterbitkan: | PAGEPress Publications 2014-03-01 |
Deskripsi
During the wild boars hunting season (2012-2013), 6508 adults were shot in Lazio Region, Viterbo territory. Out of the 6508, 393 subjects were sampled to be tested for the detection of <em>Salmonella</em> spp., <em>Yersinia enterocolitica</em>, <em>E. coli</em> VTEC and <em>Trichinella</em> spp. For this purpose, diaphragm and skeletal muscles samples were collected after the shooting, and tested at the Istituto Zooprofilattico Sperimentale Lazio and Toscana laboratories, Rome, Italy. Samples were tested respectively by polymerase chain reaction, enzyme linked immunofluorescent assay, microbiological and parasitological assays. The positivity percentages are 3.6% for <em>Salmonella</em> spp., 14.8% (out of 230 samples tested) for <em>Yersinia enterocolitica</em>, 4.6% for <em>E.coli</em> VTEC (vtx 1 and 2 genes). Each positive sample for vtx 1 and 2 was tested to isolate Verotoxigenic <em>E.coli</em> strains; none of them was positive for the same virulence genes. All diaphragm samples were negative to the trichinoscopic test. Every bacterial strain was identified serologically by seroagglutination test.