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Similarity Analysis of <i>Klebsiella pneumoniae</i> Producing Carbapenemases Isolated from UTI and Other Infections
oleh: Agata Pruss, Paweł Kwiatkowski, Monika Sienkiewicz, Helena Masiuk, Agnieszka Łapińska, Barbara Kot, Zuzanna Kilczewska, Stefania Giedrys-Kalemba, Barbara Dołęgowska
Format: | Article |
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Diterbitkan: | MDPI AG 2023-07-01 |
Deskripsi
<i>Klebsiella pneumoniae</i> is an important opportunistic pathogen responsible for severe infections, mainly urinary tract infections (UTIs) and pneumonia. Hospital epidemic infections caused by multiresistant strains of carbapenemase-producing <i>K. pneumoniae</i> are the most concerning. NDM-producing strains are resistant to a wide range of antibiotics and have become the most significant threat. Determining the natural reservoirs and routes of infections is essential to end hospital outbreaks. Understanding the relatedness of <i>K. pneumoniae</i> strains is essential to determine the range and nature of the infection. The study compared phylogenetic relatedness between multiresistant <i>K. pneumoniae</i> strains isolated from hospitalized patients. Susceptibility to drugs and mechanisms of resistance were confirmed using phenotypic methods. PFGE was used to analyze the relatedness between strains. We analyzed 69 <i>K. pneumoniae</i> strains from various healthcare units. The isolates were mainly identified from urine. Strains were resistant to β-lactam antibiotics with β-lactamase inhibitors, cephalosporins, and quinolones. Their susceptibility to aminoglycosides and carbapenem antibiotics was diverse. Most of the isolated strains produced New Delhi metallo-ß-lactamase (NDM). Although <i>K. pneumoniae</i> strains were classified into several genotype clusters, closely related isolates were confirmed in the same hospital’s wards, and in two hospitals in the same province.