Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT
oleh: Anastasiia L. Sivkina, Maria G. Karlova, Maria E. Valieva, Laura L. McCullough, Timothy Formosa, Alexey K. Shaytan, Alexey V. Feofanov, Mikhail P. Kirpichnikov, Olga S. Sokolova, Vasily M. Studitsky
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-01-01 |
Deskripsi
Sivkina et al. present a biochemical and biophysical characterization of the interaction of S. cerevisiae histone chaperone FACT with the nucleosome core particle. They show that FACT adopts a more open geometry in the presence of Nhp6, and together they unfold nucleosomes to an almost extended conformation, suggesting a mechanism for FACT-facilitated disassembly of nucleosomes.