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A list of the vascular flora occurring in the municipality of Empoli (province of Firenze, Tuscany) is provided. The list is based on a bibliographic analysis and on field studies carried out in the years 2018–2022. A total of 757 specific and subspecific taxa currently occur in the study area (including 117 aliens), plus 51 cultivated taxa. Azolla filiculoides Lam., Prunus cerasifera Ehrh., and Veronica filiformis Sm. should be considered as naturalized aliens in Tuscany.