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Long noncoding RNA (lncRNA) plays a crucial part in all kinds of life activities, especially in myogenesis. <i>SMARCD3</i> (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily d, member 3) is a member of the SWI/SNF protein complex and was reported to be required for cell proliferation and myoblast differentiation. In this study, we identified a new lncRNA named <i>SMARCD3-OT1</i> (<i>SMARCD3</i><i>overlapping</i><i>lncRNA</i>), which strongly regulated the development of myogenesis by improving the expression of <i>SMARCD3X4</i> (<i>SMARCD3</i><i>transcripts</i><i>4</i>). We overexpressed and knockdown the expression of <i>SMARCD3-OT1</i> and <i>SMARCD3X4</i> to investigate their function on myoblast proliferation and differentiation. Cell experiments proved that <i>SMARCD3-OT1</i> and <i>SMARCD3X4</i> promoted myoblast proliferation through the <i>CDKN1A</i> pathway and improved differentiation of differentiated myoblasts through the <i>MYOD</i> pathway. Moreover, they upregulated the fast-twitch fiber-related genes and downregulated the slow-twitch fiber-related genes, which indicated that they facilitated the slow-twitch fiber to transform into the fast-twitch fiber. The animals’ experiments supported the results above, demonstrating that <i>SMARCD3-OT1</i> could induce muscle hypertrophy and fast-twitch fiber transformation. In conclusion, <i>SMARCD3-OT1</i> can improve the expression of <i>SMARCD3X4</i>, thus inducing muscle hypertrophy. In addition, <i>SMARCD3-OT1</i> can facilitate slow-twitch fibers to transform into fast-twitch fibers.