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With the advent of the information age, online education has developed rapidly. The teaching reform of translation online course for business English majors in application-oriented universities is an important means to cultivate high-quality application-oriented talents. In view of this need, this study has explored the teaching reform of translation online course for business English majors in application-oriented universities from the aspects of curriculum orientation and planning, teacher team construction, curriculum resources, teaching and management as well as teaching effect. Therefore, this study aims to improve the training quality of application-oriented talents, innovate the talent training mode and form teaching characteristics.