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In this study, we demonstrate a facile approach for the synthesis of strontium tungstate (G-SrWO4) using Pomelo leaf extract via the sonochemical method. The electrocatalytic performance of G-SrWO4 solubilized Nafion (Nf) (G-SrWO4/Nf) modified GC electrode was evaluated for uric acid (UA) detection. The characteristic properties of the prepared G-SrWO4 nanocomposite were analyzed by techniques like XRD, FT-IR, FESEM, EDX, HR-TEM, SEAD, XPS, and TGA analysis. The G-SrWO4/Nf modified GC electrode displayed excellent electrocatalytic performance in UA sensing. The amperometric studies revealed that the G-SrWO4/Nf modified GC electrode performed admirably by attaining a wide linear range response of UA 0.001 μM to 0.5 μM with a limit of detection 33ppm and sensitivity of 486 μAμM−1cm−2. Meanwhile, the G-SrWO4/Nf modified GC electrode exhibited good selectivity, rapid and stable response towards UA. The practical applicability of the sensor was successfully tested by performing UA detection in the urine and serum samples with acceptable recovery results.