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Total Volatile Basic Nitrogen (TVB-N) and Trimethylamine Nitrogen (TMA-N) are the most important freshness indicators in fish. The present report demonstrates that the Gymnorhynchus gigas plerocercoid may interfere on TVBN and TMA-N determination. The study was carried-out on 101 samples: 37 Mp (muscle portions around the parasite), 32 P (larvae of Gymnorynchus gigas extracted from muscle) and 32 Mnp (muscle portions parasite-free), obtained from 10 specimens of Lepidopus caudatus kept at several temperature for different time. The TVB-N and TMA-N contents of each sample were measured using the Conway microdiffusion method. Statistically significant differences (p < 0.05) between Mp and Mnp for TMA-N were found from 24h of storage at 4 and 15°C. No significant differences (p < 0.05) between Mp and Mnp for TVB-N were observed from 48h of storage at 4 and 15°C. TVB-N and TMA-N content was variable in larvae.