Find in Library
Search millions of books, articles, and more
Indexed Open Access Databases
Study on the Preparation and Effect of Tomato Seedling Disease Biocontrol Compound Seed-Coating Agent
oleh: Yao Zhang, Yingying Li, Sibo Liang, Wei Zheng, Xiuling Chen, Jiayin Liu, Aoxue Wang
Format: | Article |
---|---|
Diterbitkan: | MDPI AG 2022-06-01 |
Deskripsi
Tomato damping-off and root rot are the two most common diseases of tomatoes at the seedling stage. At present, biological compound seed-coating agents are gradually replacing chemical agents in preventing and controlling plant diseases and insect pests, regulating plant growth, and ensuring crop yields. In this study, five biocontrol bacteria (<i>Bacillus amyloliquefaciens</i> (Ba), <i>Bacillus subtilis</i> (Bs wy-1), <i>Bacillus subtilis</i> (WXCDD105), <i>Pseudomonas fluorescens</i> (WXCDD51), and <i>Bacillus velezensis</i> (WZ-37)), with broad antibacterial spectra were mixed with auxiliary factors (inactive components of seed-coating agent) after fermentation to compound a seed-coating agent. In this study, the formula for a compound seed-coating agent was selected through orthogonal experiment. Gaseous silica was used as a thickener, and gum arabic and sodium dodecylbenzene sulfonate were used as a film-forming agent and dispersant, respectively. The mass of fumed silica, gum arabic, sodium dodecylbenzene sulfonate, and pearlescent powder was 1.3 g, 1 g, 0.05 g, and 0.5 g, respectively. Adding gibberellin can improve the ability of seed-coating agents to promote seed germination and plant growth. This showed high efficiency in preventing and controlling seedling diseases and promoting seedling growth. After 6 days of inoculation with <i>Pythium aphanidermatum</i>, which caused tomato damping-off disease, the seedling mortality rate was 26.7% lower than that of the sterile water control, and 20% lower than that of carbendazim. After 21 days of inoculation with <i>Fusarium</i> sp., which caused tomato root rot disease, the seedling mortality rate was 44.31% lower than that of the control, and 22.36% lower than that of carbendazim. The plant height, stem diameter, root length, fresh weight, and dry weight of tomato seeds treated with biological compound seed-coating agent were significantly higher than that of the control. We tested the shelf life of the biological compound seed-coating agent, and found that the effect of seed germination and radicle growth did not decrease. This research provides information on the production technology and application of biological seed-coating agents in tomato production.