Generation of a human embryonic stem cell (WAe001-A-47) with hVGLL4 doxycyclin-inducible expression by the PiggyBac transposon system
mā: Yingyi Quan, Tianci Zhang, Huan Zhang, Jiwen Yang, Peifeng Jin, Jianshe Ma, Yanyan Fan, Junming Fan, Xiaofang Fan, Yongsheng Gong, Ming Li, Yongyu Wang
| Hōputu: | Article |
|---|---|
| I whakaputaina: | Elsevier 2021-01-01 |
Whakaahuatanga
VGLL4 is a new component of the Hippo pathway and bind TEADs to compete with YAP, so as to inhibit tumor progression, but its role in stem cell and organ regeneration remains unclear. Using the PiggyBac transposon system, we generated a VGLL4 doxycycline-inducible expression human embryonic stem cell line (WAe001-A-47). The established hESC line retains its normal morphology and pluripotency markers with in vitro differentiation potential, as well as a normal karyotype.