tert-Butylphenolic Derivatives from Paenibacillus odorifer—A Case of Bioconversion

oleh: Thi-Bach-Le Nguyen, Olivier Delalande, Isabelle Rouaud, Solenn Ferron, Laura Chaillot, Rémy Pedeux, Sophie Tomasi

Format: Article
Diterbitkan: MDPI AG 2018-08-01

Deskripsi

Two compounds (1) and (2) containing tert-butylphenol groups were, for the first time, produced during the culture of Paenibacillus odorifer, a bacterial strain associated with the crustose lichen, Rhizocarpon geographicum. Their entire structures were identified by one-dimensional (1D) and two-dimensional (2D) NMR and high-resolution electrospray ionisation mass spectrometry (HRESIMS) spectroscopic analyses. Among them, Compound 1 exhibited significant cytotoxicity against B16 murine melanoma and HaCaT human keratinocyte cell lines with micromolar half maximal inhibitory concentration (IC50) values. Furthermore, after supplementation studies, a putative biosynthesis pathway was proposed for Compound 1 throughout a bioconversion by this bacterial strain of butylated hydroxyanisole (BHA), an antioxidant polymer additive.