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<p>Abstract</p> <p>A new malonamide fluoroionophore possessing two pyrene moieties was synthesized. This bispyrene exhibited the fluorescence of the pyrene monomer (λ_em= 395 nm) and intramolecular excimer (λ_em= 467 nm) emissions. The designed derivative showed the excellent ion sensing ability to Cu²⁺. The "on-off-off" and "off-on-off" fluorescence responses were demonstrated by the addition of the variable Cu²⁺concentration. The utilization of the dual off/on responses could apply to the estimation of the rough Cu²⁺concentration.</p>