A thiol probe for measuring unfolded protein load and proteostasis in cells
oleh: Moore Z. Chen, Nagaraj S. Moily, Jessica L. Bridgford, Rebecca J. Wood, Mona Radwan, Trevor A. Smith, Zhegang Song, Ben Zhong Tang, Leann Tilley, Xiaohong Xu, Gavin E. Reid, Mahmoud A. Pouladi, Yuning Hong, Danny M. Hatters
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-09-01 |
Deskripsi
Proteostasis is maintained through a number of molecular mechanisms, some of which function to protect the folded state of proteins. Here the authors demonstrate the use of TPE-MI in a fluorigenic dye assay for the quantitation of unfolded proteins that can be used to assess proteostasis on a cellular or proteome scale.