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Purpose: Determining library and information student’s familiarity with theoretical and basic subjects of discipline and attitude toward it and examining relationship between them. Methodology: Population consisted of undergraduate students of Library and Information Science of ShahidChamran University, University of Tehran, and Ferdowsi University of Mashhad studying in academic year 2010- 2011. The sample of study consisted of 108 students. The data collection tool was questionnaire with questions based on Likert five-value scale. Data was analyzed by SPSS software using statistical t-test and Pearson correlation. Findings: State of familiarity with theoretical and basic subjects of discipline of forth year students were better than the third year students. Student’s attitude toward their discipline was positive. Also, there was a strong correlation between status of familiarity with theoretical and basic subjects of discipline and attitude toward it. Originality: Findings could to help educators of library and information science to identify Student’s weaknesses regarding familiarity with theoretical and basic subjects of discipline and positive attitude toward it and try to improve them.