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Edible <i>Macadamia</i> is one of the most important commercial nut trees cultivated in many countries, but its large tree size and long juvenile period pose barriers to commercial cultivation. The short domestication period and well-annotated genome of <i>Macadamia integrifolia</i> create great opportunities to breed commercial varieties with superior traits. Recent studies have shown that members of the phosphatidylethanolamine binding protein (PEBP) family play pivotal roles in regulating plant architecture and flowering time in various plants. In this study, thirteen members of <i>MiPEBP</i> were identified in the genome of <i>M. integrifolia</i>, and they are highly similarity in both motif and gene structure. A phylogenetic analysis divided the <i>MiPEBP</i> genes into three subfamilies: <i>MFT-like</i>, <i>FT-like</i> and <i>TFL1-like</i>. We subsequently identified two <i>TERMINAL FLOWER 1</i> homologues from the <i>TFL1-like</i> subfamily, <i>MiTFL1</i> and <i>MiTFL1-like</i>, both of which were highly expressed in stems and vegetative shoots, while <i>MiTFL1-like</i> was highly expressed in young leaves and early flowers. A subcellular location analysis revealed that both <i>MiTFL1</i> and <i>MiTFL1-like</i> are localized in the cytoplasm and nucleus. The ectopic expression of <i>MiTFL1</i> can rescue the early-flowering and terminal-flower phenotypes in the <i>tfl1–14</i> mutant of <i>Arabidopsis thaliana</i>, and it indicates the conserved functions in controlling the inflorescence architecture and flowering time. This study will provide insight into the isolation of <i>PEBP</i> family members and the key targets for breeding <i>M. integrifolia</i> with improved traits in plant architecture and flowering time.