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Abstract In this paper, we deal with the inverse problem of identifying the unknown source of time-fractional diffusion equation on a columnar symmetric domain. This problem is ill-posed. Firstly, we establish the conditional stability for this inverse problem. Then the regularization solution is obtained by using the Tikhonov regularization method and the error estimates are derived under the a priori and a posteriori choice rules of the regularization parameter. Three numerical examples are presented to illustrate the validity and effectiveness of our method.