Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
oleh: Hannes Uchtenhagen, Cliff Rims, Gabriele Blahnik, I-Ting Chow, William W. Kwok, Jane H. Buckner, Eddie A. James
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-08-01 |
Deskripsi
Detection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood samples.