Find in Library
Search millions of books, articles, and more
Indexed Open Access Databases
Engineered Fungus <i>Thermothelomyces thermophilus</i> Producing Plant Storage Proteins
oleh: Larissa Balabanova, Aleksandra Seitkalieva, Yulia Yugay, Tatiana Rusapetova, Lubov Slepchenko, Anna Podvolotskaya, Margarita Yatsunskaya, Elena Vasyutkina, Oksana Son, Liudmila Tekutyeva, Yury Shkryl
Format: | Article |
---|---|
Diterbitkan: | MDPI AG 2022-01-01 |
Deskripsi
An efficient <i>Agrobacterium</i>-mediated genetic transformation based on the plant binary vector pPZP-RCS2 was carried out for the multiple heterologous protein production in filamentous fungus <i>Thermothelomyces</i> <i>thermophilus</i> F-859 (formerly <i>Myceliophthora</i> <i>thermophila </i>F-859). The engineered fungus <i>Th.</i> <i>thermophilus</i> was able to produce plant storage proteins of <i>Zea</i> <i>mays</i> (α-zein Z19) and <i>Amaranthus</i> <i>hypochondriacus </i>(albumin A1) to enrich fungal biomass by valuable nutritional proteins and improved amino acid content. The mRNA levels of <i>z19</i> and <i>a1</i> genes were significantly dependent on their driving promoters: the promoter of tryptophan synthase (P<i>trpC</i>) was more efficient to express <i>a1</i>, while the promoter of translation elongation factor (P<i>tef</i>) provided much higher levels of <i>z19 </i>transcript abundance. In general, the total recombinant proteins and amino acid contents were higher in the P<i>tef</i>-containing clones. This work describes a new strategy to improve mycoprotein nutritive value by overexpression of plant storage proteins.