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Multidrug-resistant bacteria are becoming the leading cause of death globally due to their resistance to many currently used antibiotics. Bacteria naturally have intrinsic resistance or acquired resistance to certain commonly used antibiotics. Therefore, searching for novel compounds has become necessary. <i>Trigonella foenumgraecum</i> extract was evaluated for antimicrobial and antibiofilm activities against multidrug-resistant bacteria <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The minimum inhibitory concentration and minimum bactericidal concentration of the extract were also determined. Moreover, gas chromatography-mass spectrometry (GC-MS) analysis was used to identify the phytochemical components present in the extract. GC-MS analysis revealed that <i>T. foenumgraecum</i> extract contains major compounds such as Phenol, 2-methoxy-3-(2-propenyl)-, n-Hexadecanoic acid, and 9,12,15-Octadecatrienoic acid. Both bacterial strains showed resistance to some of the antibiotics tested. <i>T. foenumgraecum</i> showed inhibitory activity against the tested bacterial strains with a MIC of 500 µg/mL and MBC of 1000 µg/mL. The methanol extract decreased the biofilm activity of both <i>E. coli</i> and <i>S. aureus</i> below the sub-minimum inhibitory concentration. The extract showed antibacterial and antibiofilm activity against the tested bacterial pathogens.