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Internal Combustion (IC) engines are commonly used in the process industry, and proper Air-Fuel Ratio (AFR) regulation in their fuel system is essential for improved engine performance, fuel efficiency, and environmental protection. Since faults in the sensors of the AFR system cause the engine to shut down, fault tolerance is essentially required for them. In this paper, an advanced Active Fault-Tolerant Control System (AFTCS) for AFR control of an IC engine is proposed to prevent the engine shutdown. For analytical redundancy, the proposed AFTCS uses the Genetic Algorithm (GA) based observer model in the Fault Detection and Isolation (FDI) unit to generate estimated value for the faulty sensors using the values of other healthy sensors. The proposed framework was simulated in the MATLAB/Simulink environment to verify its effectiveness. In comparison to previous literature works, the results show that the AFR control system has superior fault tolerance output for sensor faults, especially for the MAP sensor, in terms of less oscillatory response. Finally, a comparison of the proposed GA-based AFTCS was carried out with the existing literature works and its superior performance was elaborated.