R26R-GR: a Cre-activable dual fluorescent protein reporter mouse.
oleh: You-Tzung Chen, Ming-Shian Tsai, Tsung-Lin Yang, Amy Tsu Ku, Ke-Han Huang, Cheng-Yen Huang, Fu-Ju Chou, Hsiang-Hsuan Fan, Jin-Bon Hong, Shuo-Ting Yen, Wei-Le Wang, Chang-Ching Lin, Yu-Chen Hsu, Kang-Yi Su, I-Chang Su, Chuan-Wei Jang, Richard R Behringer, Rebecca Favaro, Silvia K Nicolis, Chung-Liang Chien, Shu-Wha Lin, I-Shing Yu
| Format: | Article |
|---|---|
| Diterbitkan: | Public Library of Science (PLoS) 2012-01-01 |
Deskripsi
Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.