Developing Oligo Probes for Chromosomes Identification in Hemp (<i>Cannabis sativa</i> L.)

oleh: Dmitry V. Romanov, Gennady I. Karlov, Mikhail G. Divashuk

Format: Article
Diterbitkan: MDPI AG 2022-07-01

Deskripsi

Hemp (<i>Cannabis sativa</i> L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of <i>C. sativa</i> have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.