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Developing Oligo Probes for Chromosomes Identification in Hemp (<i>Cannabis sativa</i> L.)
oleh: Dmitry V. Romanov, Gennady I. Karlov, Mikhail G. Divashuk
Format: | Article |
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Diterbitkan: | MDPI AG 2022-07-01 |
Deskripsi
Hemp (<i>Cannabis sativa</i> L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of <i>C. sativa</i> have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.