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Botryosphaeria dieback (BD) is an important trunk disease affecting grapevines. Several Lasiodiplodia species have been shown to be involved in BD affecting the perennial organs of grapevine, mainly causing cankers. (R)-(-)-mellein and tyrosol, two well-known fungal phytotoxins, were isolated from the organic extract of culture filtrate of Lasiodiplodia laeliocattleyae (syn. egyptiacae), which had been isolated from grapevines affected by BD in Brazil. This increases knowledge of the secondary metabolites produced by Lasiodiplodia species, confirming that (R)-(-)-mellein is a toxin typically produced by Botryosphaericeae species.