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This study was conducted to investigate the effects of composited functional bioactive substances on the growth performance and immune function of growth retardation (GR) pigs. Twenty-four 42-day-old GR pigs were fed with basal diet containing 0 or 0.5% composited functional bioactive substances package (FP) for 21 days. The results showed that dietary supplementation with FP decreased feed:gain ratio, increased the relative and absolute weight of the liver (P < 0.05). Supplementation with FP decreased serum alkaline phosphatase and malondialdehyde, but increased aspartic acid, cysteine, arginine, proline and taurine concentrations, as well as serum superoxide dismutase and catalase activities (p < 0.05). FP treatment increased all determined cytokines (IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12, GM-CSF, IFN-γ, TGF-β1, TNF-α) concentrations in serum compared with the control treatment (p < 0.05). These findings indicate that functional bioactive substances can improve feed conversion rate, serum anti-oxidant capacity and immunity and can be as effective growth promoter in the GR piglets.