Visualizing group II intron dynamics between the first and second steps of splicing
oleh: Jacopo Manigrasso, Isabel Chillón, Vito Genna, Pietro Vidossich, Srinivas Somarowthu, Anna Marie Pyle, Marco De Vivo, Marco Marcia
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-06-01 |
Deskripsi
Group II introns are self-splicing ribozymes. Here, the authors employ enzymatic assay, X-ray crystallography and molecular dynamics simulations to show that protonation of the group II intron catalytic triad plays an important role for the transition from the first to the second step of splicing.