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Aim. The aim of this study was to compare the effect of pH cycling on the microhardness of the enamel of primary human teeth treated with a conventional brown Sodium Fluoride (5% NaF) Varnish to those treated with a white Fluoride Varnish (5% NaF) enhanced with functionalized tricalcium phosphate (fTCP). Materials and Methods. Ninety extracted caries-free primary incisors were washed in a detergent and divided into three groups; group A received no treatment, teeth in group B were coated with Sodium Fluoride (5% NaF) Varnish, while teeth in group C were coated with 5% NaF varnish enhanced with functionalized tricalcium phosphate (fTCP). After ten days of pH cycling, the surface microhardness of the teeth was measured using a Knoop indenter. Results. The mean Knoop hardness number (KHN) of 5% NaF with fTCP was greater than that of 5% NaF alone while the control group had the lowest mean KHN. Conclusion. The results of this study suggest that the use of an additive such as fTCP to a fluoride varnish significantly improves the protective ability of the varnish on primary teeth in vitro.