Nuclear oligo hashing improves differential analysis of single-cell RNA-seq
oleh: Hyeon-Jin Kim, Greg Booth, Lauren Saunders, Sanjay Srivatsan, José L. McFaline-Figueroa, Cole Trapnell
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-05-01 |
Deskripsi
Using spike-in controls with current single cell RNA-seq platforms remains a challenge. Here, the authors use a mixture of short, unmodified DNA oligos as a normalization standard for sci-RNAseq to improve the detection of global transcriptome changes.