Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
oleh: Dongjie Zhu, Xiangxi Wang, Qianglin Fang, James L Van Etten, Michael G Rossmann, Zihe Rao, Xinzheng Zhang
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-04-01 |
Deskripsi
Conventional reconstruction methods used in cryo-EM single particle analysis do not take the depth of field effect into account. Here the authors present a block-based reconstruction method to deal with the depth of field effect and show that this approach can improve the resolution of cryo-EM virus structures.