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Biosynthesis of metal nanoparticles is gaining more importance owing to its simplicity, economical, sustainable route of synthesis of nanoparticles and ecofriendliness. Based on the search to improve and protect the environment by decreasing the use of toxic chemicals and eliminating biological risks in biomedical applications, the present article reports an environment friendly and unexploited methods for biofabrication of silver nanoparticles (AgNPs) using Raphanus sativus leaf extract. The synthesized AgNPs were characterized by UV-vis spectroscopy and transmission electron microscopy (TEM). The absorption spectrum of the dark brown color silver colloids showed a single and prominent peak at 431nm, indicating the presence of AgNPs. Further, catalytic degradation of methylene blue (organic dye) by using AgNPs was measured spectrophotometrically. The results revealed that biosynthesized AgNPs was found to be impressive in degrading methylene blue and can be used in water purification systems.