Find in Library

<i>Caenorhabditis elegans</i> was recently shown to be a powerful model for studying and identifying probiotics with specific functions. <i>Lactobacillus acidophilus</i> CL1285, <i>Lacticaseibacillus casei</i> LBC80R, and <i>Lacticaseibacillus rhamnosus</i> CLR2, which are three bacteria that were marketed by Bio-K+, were evaluated using the nematode <i>C. elegans</i> to study fat accumulation, lifespan, and resistance to oxidative stress. Although the general effects of probiotics in terms of protection against oxidative stress were highlighted, the CL1285 strain had an interesting and specific feature, namely its ability to prevent fat accumulation in nematodes; this effect was verified by both the Oil Red and Nile Red methods. This observed phenotype requires daf-16 and is affected by glucose levels. In addition, in a daf-16- and glucose-dependent manner, CL1285 extended the lifespan of <i>C. elegans</i>; this effect was unique to CL1285 and not found in the other <i>L. acidophilus</i> subtypes in this study. Our findings indicate that <i>L. acidophilus</i> CL1285 impacts fat/glucose metabolism in <i>C. elegans</i> and provides a basis to further study this probiotic, which could have potential health benefits in humans and/or in mammals.