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Our paper presents new aspects regarding the micropropagation of Gisela 5 cherry rootstock. In the multiplication stage, modified MS media supplemented with 0.3 and 0.5 mg/l BAP as well as two types of gelling agents were tested: 3 g/l Plant Agar and 50 g/l wheat starch. The media supplemented with 0.3 mg/l BAP and gelled with 3 g/l Plant Agar provided the highest proliferation rates (36.90 shoots/plantlet) and well-developed, high quality plantlets, whereas media gelled with starch also proved to be suitable but they caused deformations. In the in vitro rooting stage, MS media supplemented with 1 mg/l IBA provided 82.5 % rooting percentage and the plantlets were well developed. Ex vitro acclimatization of the plantlets rooted in vitro was successful in float hydroculture. Direct ex vitro rooting of shoots in floating perlite was also successful, with rooting pecentages exceeding 60 % but the plants were lesser developed as compared to the ones obtained by in vitro rooting and subsequent ex vitro acclimatization.