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Strontium oxide (SrO) is an effective catalyst for transesterification. SrO powder that is firmly deposited onto a light titanium plate (TiO₂_P), denoted as SrO/TiO₂_P, can be reinforced by forming strontium titanate (SrTiO₃) at the interface. Exposed SrO agglomerates can promote subsequent continuous transesterification process. In this work, conversion efficiency and production of biodiesel from olive oil on SrO/TiO₂_P is investigated. The as-designed SrO/TiO₂_P was followed by dip-coating and heat treatment. The physical properties of SrO/TiO₂_P were verified through ASTM D3359; the chemical structures before and after transesterification, were respectively identified by X-ray photoelectron spectroscopy and Raman spectroscopy. A focused microwave heating system was utilized for transesterification. In the optimized sample SrO/TiO₂_P (x) (x = 0.5 M), SrO firmly bonds with TiO₂_P and forms the SrTiO₃ structure. With the support of TiO₂_P, the tested oil with SrO agglomerates subsequently reacts with SrO under microwave heating. The biodiesel conversion rate reaches 87.7% after a reaction time of 4 min, while the biodiesel product has an average of 39.37 MJ/kg of combustion heat and less than 1 vol% of water content. The as-designed SrO/TiO₂_P (0.5) thus has great potential for biodiesel production and is promising with high stability in particular for a continuous fluid flow system.