Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
oleh: Peiwu Qin, Mahmut Parlak, Cem Kuscu, Jigar Bandaria, Mustafa Mir, Karol Szlachta, Ritambhara Singh, Xavier Darzacq, Ahmet Yildiz, Mazhar Adli
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-03-01 |
Deskripsi
CRISPR-Cas9 has been used to image repetitive loci in live cells to explore genome organization. Here the authors demonstrate the imaging of low-repeat regions with a single-guide RNA and the imaging of a non-repetitive region, allowing the tracking of transcriptionally active and inactive regions.