Expression analysis and single-nucleotide polymorphisms of <i>SYNDIG1L</i> and <i>UNC13C</i> genes associated with thoracic vertebral numbers in sheep (<i>Ovis aries</i>)
oleh: Y.-J. Zhong, Y. Yang, X.-Y. Wang, R. Di, M.-X. Chu, Q.-Y. Liu
| Format: | Article |
|---|---|
| Diterbitkan: | Copernicus Publications 2021-04-01 |
Deskripsi
<p>The objective of the current study was to analyze expression levels of synapse differentiation inducing 1-like (<i>SYNDIG1L</i>) and unc-13 homolog C (<i>UNC13C</i>) genes in different tissues, while single-nucleotide polymorphisms (SNPs) of two genes were associated with multiple thoracic vertebrae traits in both Small-tailed Han sheep (STH) and Sunite sheep (SNT). The expression levels of <i>SYNDIG1L</i> and <i>UNC13C</i> were analyzed in the brain, cerebellum, heart, liver, spleen, lung, kidney, adrenal gland, uterine horn, longissimus muscle, and abdominal adipose tissues of two sheep breeds with different thoracic vertebral number (TVN) sheep (T13 groups and T14 groups) by real-time quantitative polymerase chain reaction (RT-qPCR). Meanwhile, the polymorphisms of <i>UNC13C</i> gene g.52919279C<span class="inline-formula">></span>T and <i>SYNDIG1L</i> gene g.82573325C<span class="inline-formula">></span>A in T14 and T13 were genotyped by the Sequenom MassARRAY<sup>®</sup> SNP assay, and association analysis was performed with the TVN. The results demonstrated that <i>UNC13C</i> gene was extensively expressed in 11 tissues. The expression of <i>UNC13C</i> gene in longissimus muscle of T14 groups of STH was significantly higher than that of T13 groups (<span class="inline-formula"><i>P</i><0.05</span>). <i>SYNDIG1L</i> gene was overexpressed in brain and cerebellum tissues, and the expression level of <i>UNC13C</i> gene in the brain and cerebellum of T13 groups in SNT was significantly higher than that of T14 groups (<span class="inline-formula"><i>P</i><0.01</span>). Association analysis showed that SNPs found in the <i>UNC13C</i> gene had no significant effects on TVN for both two genes. The polymorphism of <i>SYNDIG1L</i> g.82573325C<span class="inline-formula">></span>A was significantly correlated with the TVN in both STH (<span class="inline-formula"><i>P</i><0.05</span>) and SNT (<span class="inline-formula"><i>P</i><0.01</span>). Taken together, the <i>SYNDIG1L</i> gene was related to thoracic vertebral development, and this variation may be potentially used as a molecular marker to select the multiple thoracic vertebrae in sheep.</p>