Evidence of a Viable but Nonculturable (VBNC) Phase in <i>B. abortus</i> S19 under Oxidative Stress (H<sub>2</sub>O<sub>2</sub>, -Fe<sup>2+</sup>, Bleach) and under Non-Oxidative Inhibitory Conditions (Isopropanol, Erythritol, Selenite)
oleh: Jens Jacob
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2024-02-01 |
Deskripsi
The effect of oxidative stress on the survival of various <i>Brucella</i> species has not been fully investigated yet. We here conducted a study in which we investigated the effect of different types of oxidative stress (Fe<sup>2+</sup>, H<sub>2</sub>O<sub>2</sub>, bleach) versus non-oxidative inhibitory effects (selenite, erythritol, and isopropanol) on the survival of <i>B. abortus</i> S19, <i>B. abortus</i> S19 ∆mglA 3.14, and <i>B. neotomae</i> 5K33. The work focuses on the appearance of ATP–CFU quotient imbalances indicating the existence of a viable but nonculturable (VBNC) form of <i>B. abortus</i> S19, as has previously been shown.