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Summary: Live imaging is an important tool to track dynamic processes such as neuronal patterning events. Here, we describe a protocol for time-lapse microscopy analysis using neuronal migration and dendritic growth as examples. This protocol can provide detailed information for understanding cellular dynamics during postembryonic development in Caenorhabditis elegans (C. elegans).For complete details on the use and execution of this protocol, please refer to Feng et al. (2020), Li et al. (2021), and Wang et al. (2021).