Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation
oleh: Emilie Viennois, Anika Tahsin, Didier Merlin
| Format: | Article |
|---|---|
| Diterbitkan: | Bio-protocol LLC 2018-05-01 |
Deskripsi
We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium chloride.