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The synthesis of novel nanomedicines through eco-friendly protocols has been applied on a large scale with the prediction of discovering alternate therapies. The current work attained phytogenic synthesis of Ag-mNPs, AgSeO₂-bmNPs, and Ag-TiO₂-bmNPs through bio-reduction using an aqueous extract of <i>Beta vulgaris</i> (red beetroot). The phytochemical profile of the eco-friendly synthesized metallic/bimetallic nanoparticles was studied. The optical properties of nano-solutions were studied via UV-visible spectroscopy. The Fourier-transform infrared spectroscopy (FT-IR) spectral analyses revealed that stretching vibrations at wavenumbers 3303.81–3327.81 cm⁻¹ attributed to phenolic hydroxyl groups documented shifts in the values in this range owing to proton dissociation through the bio-reduction of the metal ions. The surface morphology and the charge of the nanoparticles were investigated using a Transmission Electron Microscope (TEM) and zeta potential analyses. The prepared nano-solutions showed lower antioxidant activity (1,1-Diphenyl-2-picrylhydrazyl (DPPH^•) and phosphomolybdate assays) than the plant extract. These results together with phytochemical analyses support the participation of the reactive species (phenolic contents) in the bio-reduction of the metal ions in the solutions through the formation of metallic/bimetallic nanoparticles. Ag-mNPs, AgSeO₂-bmNPs, and Ag-TiO₂-bmNPs showed antibacterial potentiality. AgSeO₂-bmNPs were superior with inhibitory zone diameters of 34.7, 37.7, 11.7, and 32.7 mm against <i>Enterococcus faecalis</i>, <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, and <i>Salmonella enterica</i>, respectively. Applying the Methylthiazole Tetrazolium (MTT) assay, the Ag-TiO₂ bmNPs revealed potent cytotoxicity against the HePG2 tumor cell line (IC₅₀ = 18.18 ± 1.5 µg/mL), while Ag-SeO₂ bmNPs revealed the most potent cytotoxicity against the MCF-7 cell line (IC₅₀ = 17.92 ± 1.4 µg/mL).