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Pot trial was aimed to investigate an effect of fertilizer nitrogen on the content of vitamin C and offtake of certain macroelements by Brassica pekinensis. The experiment was realized in the years 2000 and 2001 in pots containing 10 kg of loamy brownsoil. Optimalization of NPK rates contributed to the increase of N, P, K, Mg and S offtake by the yield of final product in comparison with unfertilized control. The most marked increase offtake of nitrogen (by 14,6%) was obtained when fertilizer N was applied in the diveded form (80% N prior to seeding + 20% N in initial phase of heads formation, nitrogen applied as DAM 390). Positive effect of full NPK nutrition on the vitamin C content was determined. The highest content of vitamin C (629.0 mg.kg-1 of fresh mass) was determined in treatment where nitrogen was applied in the divided rate.