Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

oleh: Thor Vinícius Martins Fajardo, Monique Bezerra Nascimento, Marcelo Eiras, Osmar Nickel, Gilvan Pio-Ribeiro

Format: Article
Diterbitkan: Universidade Federal de Santa Maria 2015-12-01

Deskripsi

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.