Molecular Characterization of the <i>Dwarf53</i> Gene Homolog in <i>Dasypyrum Villosum</i>

oleh: Mikhail Bazhenov, Anastasiya Chernook, Pavel Kroupin, Gennady Karlov, Mikhail Divashuk

Format: Article
Diterbitkan: MDPI AG 2020-02-01

Deskripsi

The <i>Dwarf53 (D53)</i> gene, first studied in rice, encodes a protein that acts as a repressor of the physiological response of plants to strigolactones&#8212;substances that regulate the activity of axillary buds, stem growth, branching of roots and other physiological processes. In this work, we isolated and sequenced the homolog of the <i>D53</i> gene in several accessions of the wild grass <i>Dasypyrum villosum</i> of different geographical origins, resulting in the discovery of large allelic variety. A molecular marker was also created that allows us to differentiate the <i>D. villosum D53</i> gene from common wheat genes. Using this marker and monosomic addition, substitution and translocation wheat lines carrying the known <i>D. villosum</i> chromosomes, the <i>D53</i> gene was localized on the long arm of the 5V chromosome.