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The genus Latibulus Gistel, 1848 (Hymenoptera: Ichneumonidae) is taxonomically reviewed in Iran. Specimens were collected using Malaise traps in the Isfahan province, during 2013–2015. Two species, Latibulus argiolus (Rossi, 1790) (spring form) and Latibulus orientalis (Horstmann, 1987) (summer form) are identified, of which L. orientalis is a new record for the fauna of Iran. In addition, L. argiolus is recorded from central part of Iran (Isfahan) for the first time. The geographical distribution of the recorded species in relation to the overall knowledge in the target area and adjacent regions is also discussed.