Oligonucleotide Array and VITEK Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Species Identification of Blood Yeast Isolates
oleh: Ming-Chi Li, Ming-Chi Li, Tsung C. Chang, Hung-Mo Chen, Chi-Jung Wu, Chi-Jung Wu, Shu-Li Su, Susan S.-J. Lee, Susan S.-J. Lee, Po-Lin Chen, Po-Lin Chen, Po-Lin Chen, Nan-Yao Lee, Nan-Yao Lee, Nan-Yao Lee, Ching-Chi Lee, Chia-Wen Li, Chia-Wen Li, Ling-Shan Syue, Ling-Shan Syue, Wen-Chien Ko, Wen-Chien Ko, Wen-Chien Ko
| Format: | Article |
|---|---|
| Diterbitkan: | Frontiers Media S.A. 2018-01-01 |
Deskripsi
We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.