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This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. <i>Staphylococcus</i> species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. <i>mecA</i> detection and staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The <i>ccrC</i> and class-C2 <i>mec</i>, which were SCC<i>mec</i> type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCC<i>mec</i> type V was found in <i>S. aureus</i>, <i>S. intermedius</i> group, <i>S. lentus</i>, <i>S. xylosus,</i> and <i>S. arlettae</i>. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCC<i>mec</i> MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.